DOPAMINE-1 RECEPTOR SUBTYPE (D1A AND D1B) EXPRESSION IN MICRODISSECTED RAT NEPHRON SEGMENTS. • 1703
نویسندگان
چکیده
منابع مشابه
Selective inhibition of the renal dopamine subtype D1A receptor induces antinatriuresis in conscious rats.
Both dopamine D1-like (D1A and D1B) and D2-like (D2, D3, and D4) receptor subfamilies are present in the kidney. Blockade of the intrarenal D1-like receptor family is associated with natriuresis and diuresis. Because the D1A and D1B receptor subtypes are not distinguishable by currently available dopaminergic agents, their functional role remains undefined. In the present study, the effect of s...
متن کاملHigh agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype.
Dopamine D1A and D1B receptor subtypes belong to the superfamily of G protein-coupled receptors. Both receptors are coupled to the activation of adenylyl cyclase and exhibit distinct brain distribution. To identify functional differences, binding and stimulation of adenylyl cyclase were assessed in 293 cells expressing transiently either dopamine D1A or D1B receptors. Membranes expressing D1B r...
متن کاملNephron Segments of Rat Kidney Cortex
Renal cortical tubules consist of polarized epithelial cells where Na/H antiport activity has been demonstrated on the apical and/or basolateral membrane. Apical Na/H antiport activity plays an important role in transcellular bicarbonate (HCO3) reabsorption, whereas basolateral Na/H antiport activity could be involved in transcellular HCO3secretion as well as cell volume and pH control. To dete...
متن کاملMicroassay of 5'-nucleotidase and adenosine deaminase activity in microdissected nephron segments.
The present study describes a new method for microassay of the activity of 5'-nucleotidase (5'-ND) and adenosine deaminase (ADA) in the microdissected nephron segments. The nephron segments including glomeruli, proximal convoluted and straight tubules (PCT and PST), cortical and medullary thick ascending limbs, and cortical and medullary collecting ducts were microdissected. 5'-ND and ADA in th...
متن کاملQuantifying porphobilinogen deaminase mRNA in microdissected nephron segments by a modified RT-PCR.
BACKGROUND Quantifying mRNA levels by reverse transcription-polymerase chain reaction (RT-PCR), although widely exercised, is still difficult. METHODS A modified quantitative RT-PCR in which genomic DNA was used as standard was developed. The quantity of mRNA was expressed as the ratio of the PCR product from cDNA and that from genomic DNA (CG ratio). Nephron distribution of porphobilinogen d...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1997
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199704001-01722